In a schadenfreude-inducing letter, Rhode spells out the potential fines for Shkreli, who was convicted of securities fraud earlier this year.
Additionally, there was mention of Shkreli's copy of what may be "Tha Carter V", the lost double-album from rap legend Lil Wayne.
The letter identifies specific assets to be forfeited, including the famous "Once Upon a Time In Shaolin", the only extant copy of a Wu Tang Clan album Shkreli purchases and famously bragged about on social media.
The government said in its submission Friday it may also seek a Picasso painting Shkreli owns, his Enigma machine from World War II and $5 million from his E-Trade brokerage account.
Prosecutors said that, after a "diligent" search to locate "traceable assets" without success, they were forced to turn to substitute assets that could be seized to make up the balance.More news: OSU coach Mike Gundy has phone conversations with Tennessee
Shkreli is best known for raising the price of Daraprim, a then-62-year-old drug frequently used to treat HIV patients, from $13.50 to $750 a pill.
It's unclear whether Shkreli is still the owner. A judge revoked his bail in September after he publicly offered $5,000 for a lock of Hillary Clinton's hair. Prosecutors said the post showed he was a threat to public safety.
Assistant U.S. Attorney Claire Kedeshian calls the figure a "conservative computation" of the amount Shkreli should be made to forfeit after he was found guilty in August of three counts. "Accordingly, forfeiture of any assets is not an appropriate remedy", he said in a statement.
Shkreli's trial posed an unusual obstacle for prosecutors in that investors who were defrauded did not lose money.
The case is U.S. v. Shkreli, 15-cr-00637, U.S. District Court, Eastern District of New York (Brooklyn).